Thermo Scientific™

Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit

Catalog number:  32054

Related applications:

Cellular Imaging

| Protein Electrophoresis & Western Blotting

Back to top

Description

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Standard Kits contain only the two essential reagents (avidin and biotinylated HRP) can be used with nearly any species of biotinylated secondary antibody. The Ultra-Sensitive ABC Peroxidase Staining Kits are approximately five times more sensitive than the regular-senstivity kit, enabling more dilute primary antibodies to be used while producing equal staining intensity. Ultra-Sensitive Kits are offered as a Standard Kit and as Complete Kits, which contain biotinylated secondary antibodies and appropriate block serum for use with mouse or rabbit antibodies, respectively.

Related Products
ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Biotin-Based,Chromogenic,Colorimetric
Product Line: Pierce™
Product Size: 8 mL
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Technique: IHC (Immunohistochemistry),Immunodetection

Contents & storage

Sufficient For: At least 10 mL of ABC staining solution
• Reagent A (Avidin), 2 mL
• Reagent B (Biotinylated HRP), 2 mL
• Blocking Serum (goat), 3 mL
• Biotinylated Goat Anti-Rabbit IgG, 1 mL
• Mixing bottles with dropper dispenser tips, 3

Store all reagents at 4°C