Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.
Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Standard Kits contain only the two essential reagents (avidin and biotinylated HRP) can be used with nearly any species of biotinylated secondary antibody. The Ultra-Sensitive ABC Peroxidase Staining Kits are approximately five times more sensitive than the regular-senstivity kit, enabling more dilute primary antibodies to be used while producing equal staining intensity. Ultra-Sensitive Kits are offered as a Standard Kit and as Complete Kits, which contain biotinylated secondary antibodies and appropriate block serum for use with mouse or rabbit antibodies, respectively.
For Research Use Only. Not for use in diagnostic procedures.
HRP (Horseradish Peroxidase) Substrate
Contents & storage
Sufficient For: At least 10 mL of ABC staining solution
• Reagent A (Avidin), 2 mL
• Reagent B (Biotinylated HRP), 2 mL
• Blocking Serum (horse), 3 mL
• Biotinylated Horse Anti-Mouse IgG, 1 mL
• Mixing bottles with dropper dispenser tips, 3