Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).
• AP substrate—for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples • Chromogenic—no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph • Package options—choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor
Highlights of Pierce NBT/BCIP 1-Step Solution (Part No. 34042): • Best for immunoblotting applications • Ready-to-use single component • Sensitive with low background and minimial assay-to-assay variability
Highlights of Pierce NBT/BCIP plus Suppressor 1-Step Solution (Part No. 34070): • Best for immunohistochemistry applications • Ready-to-use single component • Contains 1 mM levamisole to inhibit endogenous phosphatase activity, while yielding an intense dark purple precipitate in the presence of calf intestinal phosphatase