1-Step™ PNPP Substrate Solution

Catalog number: 37621

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. This product is a ready-to-use (1X) proprietary buffered solution of PNPP.

Features of PNPP (p-nitrophenyl phosphate):

• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative

Until now, the method for preparing PNPP solutions required dissolving the powder or tablets in buffer and then diluting the solution to the desired concentration. Substrate instability made it necessary to prepare it on a daily basis. Pierce 1-Step PNPP circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2°C-8°C and can be stopped with conventional methods.

PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. The substrate is offered in several formats.

Related Products
Pierce™ PNPP Substrate Kit
PNPP Substrate Tablets
PNPP Substrate
For Research Use Only. Not for use in diagnostic procedures.


Product Size: 100 mL
Substrate Type: AP (Alkaline Phosphatase) Substrate
Detection Method: Colorimetric
Substrate Properties: Chemical Substrate

Contents & storage

Upon receipt store product at 4°C. Product is shipped at ambient temperature.


Manuals & protocols

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