What incubation time and temperature should I use for effective blocking using the SuperBlock Blocking Buffer ?
Typical procedures use 1 hour at room temperature or overnight at 4°C. However, sufficient blocking in many procedures is possible in 3 x 2 minutes at room temperature.
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Does SuperBlock Blocking Buffer work with nylon and nitrocellulose membrane?
Yes, SuperBlock Blocking Buffer works with nylon and nitrocellulose membrane.
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Does SuperBlock Blocking Buffer need to be diluted?
No. It is supplied at 1X concentration and should be used as is.
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Does SuperBlock Blocking Buffer contain albumin, biotin, DNA, detergent or phosphoproteins?
No. Unless it includes T20 in the name, for example, SuperBlock T20 (PBS) Blocking Buffer, which contains 0.05% Tween 20.
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