Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a ready-to-use format.
Features of Protein-Free Blocking Buffer: • Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers • Applications—western blotting (membranes), ELISA (microplates), and arrays (coated glass slides) • Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems • High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)
Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.