Thermo Scientific™

PNPP Substrates

Catalog number: 37620
Thermo Scientific™

PNPP Substrates

Catalog number: 37620
PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.
 
Catalog Number
37620
Unit Size
105 tablets
Description
PNPP Substrate Kit
Price (USD)
Price: 252.00
Your price:
Availability
-
Full specifications
Quantity
DescriptionPNPP Substrate Kit
Product TypeELISA Substrate
Quantity1 Kit
Detection MethodColorimetric
Product LinePierce™
Substrate PropertiesChemical Substrate
Substrate TypeAlkaline Phosphatase
Unit Size105 tablets
Catalog Number
Unit Size
25 g
Description
PNPP Powder
Price (USD)
Price: 192.00
Your price:
Availability
-
Full specifications
Quantity
DescriptionPNPP Powder
Product TypeELISA Substrate
Quantity25 g
Detection MethodColorimetric
FormPowder
Substrate PropertiesChemical Substrate
Substrate TypeAlkaline Phosphatase
Shipping ConditionAmbient
Unit Size25 g
Catalog Number
Unit Size
105 tablets
Description
PNPP Tablets
Price (USD)
Price: 198.00
Your price:
Availability
-
Full specifications
Quantity
DescriptionPNPP Tablets
Product TypeELISA Substrate
Quantity105 Tablets
Detection MethodColorimetric
FormatTablet
Substrate PropertiesChemical Substrate
Substrate TypeAlkaline Phosphatase
Shipping ConditionWet Ice
Unit Size105 tablets
Catalog Number
Unit Size
100 mL
Description
1-Step™ PNPP Substrate Solution
Price (USD)
Price: 151.00
Your price:
Availability
-
Full specifications
Quantity
Description1-Step™ PNPP Substrate Solution
Product TypeELISA Substrate
Quantity100 mL
Detection MethodColorimetric
Product Line1-Step™
Substrate PropertiesChemical Substrate
Substrate TypeAlkaline Phosphatase
Shipping ConditionAmbient
Unit Size100 mL
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
37620Full specifications
105 tabletsPNPP Substrate Kit
Price: 252.00
Your price:
-
DescriptionPNPP Substrate Kit
Product TypeELISA Substrate
Quantity1 Kit
Detection MethodColorimetric
Product LinePierce™
Substrate PropertiesChemical Substrate
Substrate TypeAlkaline Phosphatase
Unit Size105 tablets
34045Full specifications
25 gPNPP Powder
Price: 192.00
Your price:
-
DescriptionPNPP Powder
Product TypeELISA Substrate
Quantity25 g
Detection MethodColorimetric
FormPowder
Substrate PropertiesChemical Substrate
Substrate TypeAlkaline Phosphatase
Shipping ConditionAmbient
Unit Size25 g
34047Full specifications
105 tabletsPNPP Tablets
Price: 198.00
Your price:
-
DescriptionPNPP Tablets
Product TypeELISA Substrate
Quantity105 Tablets
Detection MethodColorimetric
FormatTablet
Substrate PropertiesChemical Substrate
Substrate TypeAlkaline Phosphatase
Shipping ConditionWet Ice
Unit Size105 tablets
37621Full specifications
100 mL1-Step™ PNPP Substrate Solution
Price: 151.00
Your price:
-
Description1-Step™ PNPP Substrate Solution
Product TypeELISA Substrate
Quantity100 mL
Detection MethodColorimetric
Product Line1-Step™
Substrate PropertiesChemical Substrate
Substrate TypeAlkaline Phosphatase
Shipping ConditionAmbient
Unit Size100 mL
Showing 4 of 4

Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.

Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405 nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative

The substrate is offered in several formats:
PNPP Substrate
• Powder Form

PNPP Substrate Tablets
• 5 mg tablets with an inert filler as the balance

PNPP Substrate Kit
• PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.

1-Step™ PNPP Substrate Solution
• Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Sufficient for: 5 mL of typical 1X substrate solution per tablet (525 mL total, approx. 5250 wells at 100 μL per well)
• Diethanolamine Buffer, 225 mL
• PNPP Tablets, 105 tablets (5 mg/tablet)

Store buffer at 4°C and PNPP tablets at -20°C.

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