Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. Our Phosphatase Substrate Kit contains PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Part No. 34064) is also provided individually as a 5X concentrate. The Pierce formulation has an optimal and consistent pH and it is stable even at a 1X concentration.
Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative
-Nitrophenyl Phosphate, Disodium Salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. The substrate is offered in several formats.Related Products1-Step™ PNPP Substrate SolutionPNPP Substrate TabletsPNPP Substrate
For Research Use Only. Not for use in diagnostic procedures.