TaqMan™ Sample-to-SNP™ Kit (200 mL sample prep, 10 mL PCR)

Description

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hr. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 4,000 sample preparation reactions at 50 µL/ reaction and 4,000 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

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Specifications

Detection Method:	Primer-Probe Detection
For Use With (Equipment):	7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp™ 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti® Thermal Cycler
Genotyping Target:	SNPs (Known), SNPs (Unknown or Numerous)
Hot Start:	Built-In Hot Start
PCR Method:	qPCR
Passive Reference Dye:	ROX (Pre-mixed)
Polymerase:	AmpliTaq® DNA Polymerase
Primer-Probe Compatibility:	TaqMan®
Product Line:	Sample-to-SNP™, TaqMan™
Product Size:	1 kit
Reaction Speed:	Fast, Standard
Sample Type (General):	Blood, Buccal Samples, Cells, Plant Samples, Tissue
Sample Type (Specific):	Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Technique:	SNP Genotyping (Real-Time PCR-Based)

Contents & storage