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PrepSEQ® Nucleic Acid Extraction Kit for Food and Environmental Testing

Catalog number: 4428176

Applied Biosystems™  Related applications: DNA Extraction | Diagnostic Testing | Water Testing

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Description

The PrepSEQ® Nucleic Acid Extraction Kit produces high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples. The quantity and quality of the DNA can greatly impact the success of the sample analysis process and the overall quality of the final result. Food and environmental samples can be challenging to process because they often contain PCR inhibitors. For laboratories looking to achieve better real-time PCR results and improve overall efficiency, the PrepSEQ™ Nucleic Acid Extraction Kit meets these needs.

Automated Sample Preparation for PCR-Based Assays
• Extract high-quality bacterial DNA
• Process a wide range of food and environmental samples
• Fast time-to-results
• Prepare hundreds of samples per shift per instrument

High-Throughput Food and Environmental Sample Preparation
PCR-based bacterial assays have become an important tool in food safety and environmental testing. The Applied Biosystems PrepSEQ™ Nucleic Acid Extraction Kit prepares high-quality bacterial DNA from food and environmental samples for PCR-based testing using magnetic bead-based extraction. In combination with the automated MagMAX™ Express-96 Deep Well Magnetic Particle Processor, this optimized solution enables laboratories to prepare a wide range of sample types at rates of up to hundreds of samples per eight-hour shift.

Streamlined Workflow
An easy-to-use, easy-to-learn protocol simplifies integration of the PrepSEQ™ Nucleic Acid Extraction Kit into the laboratory workflow. The same protocol can be used on a wide range of sample types, and the MagMAX™ Express-96 instrument automates many of the workflow steps to reduce hands-on time.

A Complete Solution
The PrepSEQ™ Nucleic Acid Extraction Kit is part of a fully integrated, start-to-finish solution for food and environmental testing. The kit has been performance-verified for food and environmental testing with MicroSEQ® Microbial Detection Kits, the Applied Biosystems® 7500 Fast Real-Time PCR System, and RapidFinder™ Express Software. For research use only. Not intended for any animal or human therapeutic or diagnostic use.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.
For testing of Food and Environmental samples only.

Specifications

Sample Type (General): Liquid Samples (e.g. Serum), Bacteria, Food & Environmental Samples
Sample Type (Specific): Food, Bacteria (Gram-Positive), Any Swab Type, Bacteria (Gram-Negative), Bacterial Culture, Water Samples
Isolation Technology: Magnetic Bead, Enzymatic
Downstream Application: PCR, Real-Time Quantitative PCR (qPCR)
Final Product: Bacterial DNA, Total RNA (Bacterial)
For Use With (Equipment): MagMAX™ Express-96 Deep Well Magnetic Particle Processor
High Throughput Compatibility: Automated Protocols, High Throughput-Compatible
Format: 96-well plate
Product Size: 300 preps
Number of Reactions: 300 Reactions
Starting Material (Amount): 1 ml culture

Contents & storage

This kit contains:
• 6 bottles of Lysis Buffer
• 6 tubes of Magnetic Particles (store at 2 to 8°C)
• 3 empty bottles for binding solution
• 6 bottles of Wash Buffer Concentrate
• 3 bottles of Elution Buffer
• 3 bottles of Proteinase K Buffer
• 3 tubes of Proteinase K (store at -20°C).

Unless otherwise indicated, each component should be stored at RT.

This kit contains enough supplies for 100 extractions.

Documents

Manuals & protocols