Customer Notice: Please be sure to follow the primer concentration guidelines as specified in the User Guide. For example, if you are using SYBR® Select with a StepOne™ or StepOnePlus™ Real-Time PCR Instrument under Standard Mode, please use a primer concentration less than 200 nM. Also, for these two instruments, please note that new StepOne™ Software v2.3 is also available for download.
Applied Biosystems™

SYBR™ Select Master Mix

Catalog number: 4472920
Applied Biosystems™

SYBR™ Select Master Mix

Catalog number: 4472920
Catalog Number
4472920
Unit Size
10 x 5 mL
Price (USD)
3,215.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1 x 1 mL
Price (USD)
91.00
Your Price:
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Quantity
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Unit Size
1 x 5 mL
Price (USD)
378.00
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Unit Size
2 x 5 mL
Price (USD)
730.00
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Unit Size
5 x 5 mL
Price (USD)
1,690.00
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Quantity
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Unit Size
1 x 50 mL
Price (USD)
3,195.00
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
447292010 x 5 mL
3,215.00
Your Price:
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44729031 x 1 mL
91.00
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44729081 x 5 mL
378.00
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44729182 x 5 mL
730.00
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44729195 x 5 mL
1,690.00
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44729131 x 50 mL
3,195.00
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***
Product Overview
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Additional Information
Recommendations
SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.

Features of the SYBR® Select Master Mix include:

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity
SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR Select Master Mix is specially formulated to provide robust results at 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility
SYBR® Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments except the 7900HT real-time PCR system. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.

View performance data.

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Real Time PCR (qPCR)
For Use With (Equipment)
7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, AB StepOnePlus™, Fast Mode, AB StepOne™, Standard Mode, AB 7500, Fast Mode, AB 7500, Standard Mode, AB StepOne™, Fast Mode, AB StepOnePlus™, Standard Mode
Label or Dye
SYBR™ GreenER™
Passive Reference Dye
ROX (Pre-mixed)
Primer Probe Compatibility
Unlabeled Oligos
Product Line
SYBR™
Product Type
Real Time PCR SYBR Master Mix
Purity or Quality Grade
UP (Ultra Pure)
Quantity
10 x 5mL
Sufficient For
100 Reactions of 20 μL
Concentration
2X
Detection Method
SYBR
Form
Liquid
Format
Vial(s)
GC-Rich PCR Performance
High
Hot Start
Built-In Hot Start
No. of Reactions
5000
PCR Method
qPCR
Polymerase
AmpliTaq™ DNA Polymerase
Purity
UP (Ultra Pure)
Reaction Speed
Fast, Standard
Sample Type
DNA (Genomic), cDNA
Volume
10 x 5 mL

Contents & Storage

The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 µL reactions (10 µL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.

Figures

Frequently asked questions (FAQs)

Citations & References