Thermo Scientific™

Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kit

Catalog number:  44980

 Related applications:

Antibody Production

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The Thermo Scientific Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.

Features of the Mouse IgG1 Fab and F(ab')2 Preparation Kit:

High capacity—kit provides for fragmenting 0.25 to 4 mg IgG.
Complete—kit includes all reagents needed to prepare and purify antibody fragments
Enzyme-free digestion products—Immobilized Ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme
Optimized for mouse IgG1—the kit procedure is optimized for mouse IgG1; the only effective method for this IgG species and subclass (see

Related Products
Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit
Pierce™ F(ab')2 Preparation Kit
Pierce™ Fab Preparation Kit
For Research Use Only. Not for use in diagnostic procedures.


Product Line: Pierce™
Product Size: 1 kit

Contents & storage

Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG
• Pierce Ficin Agarose, 2.5 mL
• Cysteine-HCl, 1 g
• Mouse IgG1 Digestion Buffer, 120 mL
• NAb Protein A Plus Spin Column, 1 mL, 1 column
• Protein A Binding Buffer, 120 mL
• IgG Elution Buffer, 120 mL
• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns
• Spin Columns, 0.8 mL, 10 columns
• Microcentrifuge tubes, 2 mL, 30 tubes

Store at 4–8°C.


Manuals & protocols