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Thermo Scientific™
Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kit
Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.
| Catalog Number | Quantity |
|---|---|
| 44980 | 2.5 mL kit |
Catalog number 44980
Price (USD)
966.00
Each
Quantity:
2.5 mL kit
Price (USD)
966.00
Each
The Thermo Scientific Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.
Features of the Mouse IgG1 Fab and F(ab')2 Preparation Kit:
- High capacity—kit provides for fragmenting 0.25 to 4 mg IgG
- Complete—kit includes all reagents needed to prepare and purify antibody fragments
- Enzyme-free digestion products—Immobilized Ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme
- Optimized for mouse IgG1—the kit procedure is optimized for mouse IgG1; the only effective method for this IgG species and subclass
Related Products
- Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit (Cat. No. 44680)
- Pierce™ F(ab')2 Preparation Kit (Cat. No. 44988)
- Pierce™ Fab Preparation Kit (Cat. No. 44985)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Kit ContentsPierce Mouse IgG1 Fab and F(ab')2 Preparation Kit
Quantity2.5 mL kit
Product LinePierce
Unit SizeEach
Contents & Storage
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG
• Pierce Ficin Agarose, 2.5 mL
• Cysteine-HCl, 1 g
• Mouse IgG1 Digestion Buffer, 120 mL
• NAb Protein A Plus Spin Column, 1 mL, 1 column
• Protein A Binding Buffer, 120 mL
• IgG Elution Buffer, 120 mL
• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns
• Spin Columns, 0.8 mL, 10 columns
• Microcentrifuge tubes, 2 mL, 30 tubes
Store at 4–8°C.
• Pierce Ficin Agarose, 2.5 mL
• Cysteine-HCl, 1 g
• Mouse IgG1 Digestion Buffer, 120 mL
• NAb Protein A Plus Spin Column, 1 mL, 1 column
• Protein A Binding Buffer, 120 mL
• IgG Elution Buffer, 120 mL
• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns
• Spin Columns, 0.8 mL, 10 columns
• Microcentrifuge tubes, 2 mL, 30 tubes
Store at 4–8°C.