What are the Pierce Protein A and Protein G IgG Plus Orientation kits for?
The Orientation kits allow for binding of an antibody to the Protein A or G resin. After the antibody is bound, an amine- reactive crosslinker (DSS) is added to covalently attach the antibody to the support. This allows for the target antigen to elute free of antibody contamination. The Protein A and G Orientation Kits are used for larger-scale purification of IgG than is typical in an immunoprecipitation.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.