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Invitrogen
FAS (soluble) Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
FAS (soluble) Antibody (500-P295BT-25UG) in WB
Western Blot: To detect Human sFas Receptor by Western Blot analysis FAS (soluble) Polyclonal Antibody, Biotin (Product # 500-P295BT-500UG) can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human sFas Receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P295BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human sFas Receptor
Conjugate
Biotin
Biotin
Biotin
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sFas Receptor. Anti-Human sFas Receptor-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human sFas Receptor by sandwich ELISA (using 100 mL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Human sFas Receptor (500-P295) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sFas Receptor.
Western Blot: To detect Human sFas Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human sFas Receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P295BT-1MG will be provided as 2 x 500 µg
Target Information
CD95, also known as Fas or APO-1, is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor in the tumor necrosis factor receptor (TNFR) superfamily. It is expressed by a wide range of hematopoietic and non-hematopoietic cells, including monocytes, neutrophils, lymphocytes, and fibroblasts. CD95 also exists in a soluble form weighing approximately 26 kDa. The interaction of CD95 with its ligand, FasL, induces apoptosis, playing a crucial role in peripheral tolerance and anti-viral immune responses. Upon stimulation, CD95 leads to the aggregation of its intracellular Fas-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases. This cascade results in apoptosis. CD95 is also involved in activating NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and transduces proliferative signals in normal diploid fibroblasts and T cells. There are at least eight alternatively spliced transcript variants encoding seven distinct isoforms of CD95. Isoforms lacking the transmembrane domain may negatively regulate apoptosis mediated by the full-length isoform. The Fas/Fas ligand system is implicated in several human diseases, including AIDS, hepatitis, and cancer, highlighting its importance in immune regulation and disease pathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 774delG; 943dupG; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; apoptosis-mediating surface antigen FAS; CD95 antigen; FAS; Fas (TNF receptor superfamily, member 6); Fas AMA; FAS9; FASLG receptor; mutant tumor necrosis receptor superfamily member 6; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; TNF-like apoptosis-inducing receptor; TNFRSF6; tumor necrosis factor receptor superfamily, member 6; unnamed protein product
Gene Aliases: ALPS1A; APO-1; APT1; CD95; FAS1; FASTM; TNFRSF6
UniProt ID: (Human) P25445
Entrez Gene ID: (Human) 355
apoptotic process
activation-induced cell death of T cells
immune response
signal transduction
extrinsic apoptotic signaling pathway via death domain receptors
regulation of stress-activated MAPK cascade
tumor necrosis factor-mediated signaling pathway
cellular response to amino acid starvation
Fas signaling pathway
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
protein-containing complex assembly
cellular response to mechanical stimulus
cellular response to hyperoxia
motor neuron apoptotic process
extrinsic apoptotic signaling pathway
necroptotic signaling pathway
positive regulation of reactive oxygen species biosynthetic process
positive regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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