Thermo Scientific™

Pierce™ NeutrAvidin™ UltraLink™ Resin

Catalog number: 53150
Thermo Scientific™

Pierce™ NeutrAvidin™ UltraLink™ Resin

Catalog number: 53150
Catalog Number
53150
Unit Size
5 mL
Price (USD)
Price: 384.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
531505 mL
Price: 384.00
Your Price:
-
Product Overview
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Thermo Scientific NeutrAvidin UltraLink Resin is a beaded polyacrylamide resin of immobilized NeutrAvidin Protein, a modified form of avidin with exceptional biotin-binding characteristics for affinity purification methods.

Features of NeutrAvidin UltraLink Resin:

NeutrAvidin protein—purified, deglycosylated avidin protein (60kDa, pI 6.3); tetrameric with four biotin-binding site per molecule
UltraLink Resin—acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium-pressure applications
Standard capacity—this variety of beads has a normal load of immobilized NeutrAvidin protein, providing a binding capacity of 1 to 2 mg of biotinylated BSA per mL of resin

NeutrAvidin UltraLink Resin consists of a specially purified and deglycosylated form of avidin that has been covalently immobilized onto a beaded acrylamide support. Compared to native avidin, NeutrAvidin protein is more neutral and exhibits much less nonspecific binding properties in biotin-binding applications. UltraLink beads are hydrophilic, charge-free, highly crosslinked, rigid and durable. These properties make the affinity resin suitable for a variety of batch- or column-type affinity procedures that require medium-pressure chromatography. The resin provides an alternative to agarose supports when situations demand it. Generally, NeutrAvidin products function as well or better than their streptavidin counterparts and are less expensive.

NeutrAvidin protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. NeutrAvidin resins are prepared by covalently coupling the protein using efficient and stable chemistries, resulting in supports that are resistant to leaching and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies.

Properties of Thermo Scientific UltraLink Resin:
• Support pH Stability: 1 to 13
• Average Particle Size: 50 to 80 microns
• Exclusion Limit: greater than 2,000,000 daltons
• Average Surface Area: greater than 250m2 per gram of beads
• Average Pore Volume: greater than 1.2 mL per gram of beads (>60% of bead volume)
• Pore Size: 500 to 1000 angstroms (50 to 100nm)
• Maximum Linear Velocity: 3000 cm per hour
• Maximum Pressure: 100psi (6.9 bar)

Related Products
Pierce™ NeutrAvidin™ Agarose
Pierce™ High Capacity NeutrAvidin™ Agarose
Pierce™ NeutrAvidin™ Plus UltraLink™ Resin
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity (Metric)
1 to 2 mg Biotinylated BSA Binding per mL Resin
Description
Pierce NeutrAvidin UltraLink Resin
Particle Size
50 to 80 μm
Form
Bottle
Column Type
Affinity, UltraLink™ Resin
Product Line
NeutrAvidin™, Pierce™, UltraLink™
Quantity
5 mL
Target
Biotin-Tagged Molecule
Type
Avidin Resin
Stationary Phase
NeutrAvidin Protein
Format
Bottle

Contents & Storage

Upon receipt store at 4°C.