Pierce™ NeutrAvidin™ Plus UltraLink™ Resin

Thermo Scientific NeutrAvidin Plus UltraLink Resin is a beaded polyacrylamide resin of immobilized NeutrAvidin Protein, a modified form of avidin with exceptional biotin-binding characteristics for affinity purification methods.

Features of NeutrAvidin Plus UltraLink Resin:

• NeutrAvidin protein—purified, deglycosylated avidin protein (60kDa, pI 6.3); tetrameric with four biotin-binding site per molecule
• UltraLink Resin—acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium-pressure applications
• High capacity—this variety of beads has a dense load of immobilized NeutrAvidin protein, providing a binding capacity greater than 3 mg of biotinylated BSA per mL of resin

NeutrAvidin Plus UltraLink Resin consists of a specially purified and deglycosylated form of avidin that has been covalently immobilized at high density onto a beaded acrylamide support. Compared to native avidin, NeutrAvidin protein is more neutral and exhibits much less nonspecific binding properties in biotin-binding applications. UltraLink beads are hydrophilic, charge-free, highly crosslinked, rigid and durable. These properties make the affinity resin suitable for a variety of batch- or column-type affinity procedures that require medium-pressure chromatography. The resin provides an alternative to agarose supports when situations demand it. Generally, NeutrAvidin products function as well or better than their streptavidin counterparts and are less expensive.

NeutrAvidin protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. NeutrAvidin resins are prepared by covalently coupling the protein using efficient and stable chemistries, resulting in supports that are resistant to leaching and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies.

Properties of Thermo Scientific UltraLink Resin:
• Support pH Stability: 1 to 13
• Average Particle Size: 50 to 80 microns
• Exclusion Limit: greater than 2,000,000 daltons
• Average Surface Area: greater than 250m2 per gram of beads
• Average Pore Volume: greater than 1.2 mL per gram of beads (>60% of bead volume)
• Pore Size: 500 to 1000 angstroms (50 to 100nm)
• Maximum Linear Velocity: 3000 cm per hour
• Maximum Pressure: 100psi (6.9 bar)

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Pierce™ NeutrAvidin™ UltraLink™ Resin