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Perforin-1 (Pore Forming Protein) (Apoptosis Marker) Recombinant Mouse Monoclonal Antibody (rPRF1/8058)
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FIGURE: 1 / 2
Perforin-1 (Pore Forming Protein) (Apoptosis Marker) Antibody (5551-MSM7-P1ABX) in IHC (P)
Formalin-fixed, paraffin-embedded human spleen stained with Perforin-1 Recombinant Mouse Monoclonal Antibody (rPRF1/8058). Inset: PBS instead of primary antibody; secondary only negative control.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
5551-MSM7-P1ABX
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
rPRF1/8058
Immunogen
Recombinant human Perforin-1 protein fragment (around aa 355-555).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Positive Control:HDLM-2 cells. Human spleen.
Cellular Location: Cytoplasm.
Target Information
Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytolysin; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; perforin 1 (pore forming protein); Perforin1; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; PRF1 (pore forming protein 1); RP11-710A11.3; unnamed protein product
Gene Aliases: HPLH2; P1; PFP
UniProt ID: (Human) P14222
Entrez Gene ID: (Human) 5551
immunological synapse formation
leukocyte mediated cytotoxicity
T cell mediated cytotoxicity
defense response to tumor cell
immune system process
immune response to tumor cell
apoptotic process
defense response
cellular defense response
protein secretion
protein import
protein homooligomerization
defense response to virus
positive regulation of killing of cells of other organism
protein transmembrane transport
granzyme-mediated programmed cell death signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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