CaptureSelect™ Human Growth Hormone (HGH) RoboColumn™ Pre-packed columns

CaptureSelect RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

The CaptureSelect Human Growth Hormone (hGH) Affinity Matrix in this robocolumn has been specifically designed for the purification of human growth hormone from recombinant sources. The high selectivity and yields obtained using CaptureSelect Human Growth Hormone Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step.

Features of this affinity matrix include:

• Highly selective for recombinant hGH
• Efficient purification of recombinant hGH with high yields and retained activity
• Excellent scalability
• Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Characteristics

• Column size: 200 μL, 1 strip, 8 columns (8-row)
• Column dimension: 5-mm diameter, 10-mm bed height
• Matrix: agarose-based, epoxide-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect hGH affinity ligand
• Ligand coupling: epoxide coupling
• Binding capacity: ∼3 mg hGH/mL resin depending on flow rate, column height, and contact time
• Elution conditions: 20 mM citric acid, pH 3.0
• Formulation buffer: 20% (v/v) ethanol
• Operating pressure: 2 bar (maximum)
• Flow rate: 16–150 cm/hr

RoboColumn is a trademark of Repligen.