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FIGURE: 1 / 3
IDH2 Antibody (711748) in WB
Western blot analysis was performed on Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1) HEK-293 (Lane 2), MCF-7 (Lane 3), U-87 MG (Lane 4), LNCaP (Lane 5) and tissue extracts of Rat Kidney (Lane 6), Mouse Skeletal Muscle (Lane 7) and Mouse Heart (Lane 8). The blots were probed with Anti-IDH2 Recombinant Rabbit Superclonal™ Antibody (Product # 711748, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). 45, 5... View More
Product Details
711748
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
8 HCLC
Immunogen
Protein corresponding to human IDH2 [aa41-aa452]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Pig, Mouse.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NADas the electron acceptor and the other NADP. Five isocitrate dehydrogenases have been reported: three NAD-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ICD-M; IDH; IDP; isocitrate dehydrogenase (NADP(+)) 2, mitochondrial; isocitrate dehydrogenase 2 (NADP+), mitochondrial; Isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; NADP+-specific ICDH; NADP+-specific isocitrate dehydrogenase; Oxalosuccinate decarboxylase; unnamed protein product
Gene Aliases: D2HGA2; E430004F23; ICD-M; IDH; IDH-2; IDH2; IDHM; IDP; IDPM; mNADP-IDH
UniProt ID: (Human) P48735, (Mouse) P54071, (Rat) P56574
Entrez Gene ID: (Human) 3418, (Mouse) 269951, (Rat) 361596
carbohydrate metabolic process
glyoxylate cycle
tricarboxylic acid cycle
isocitrate metabolic process
2-oxoglutarate metabolic process
NADP metabolic process
NADP biosynthetic process
negative regulation of glial cell proliferation
negative regulation of glial cell migration
negative regulation of matrix metallopeptidase secretion
oxidation-reduction process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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