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FIGURE: 1 / 3
Phospho-EEF2 (Thr56) Antibody (711843) in WB
Western blot analysis was performed on Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), T98G (Lane 2), Hep G2 (Lane 3), 3T3-L1 (Lane 4), 3T3-L1 treated with Forskolin (100uM for 60 mins) (Lane 5), NIH/3T3 (Lane 6), NIH/3T3 treated with Forskolin (100uM for 60 mins) (Lane 7). The blots were probed with Anti-EEF2 pT56 Recombinant Rabbit Superclonal™ Antibody (Product # 711843, 2.5 µg/mL) and detected by... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
711843
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Peptide corresponding to human Phospho-EEF2 (Thr56) [aa54-aa61]
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Cat, Bovine.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; polypeptidyl-tRNA translocase; unnamed protein product
Gene Aliases: EEF-2; EF-2; EF2; SCA26
UniProt ID: (Human) P13639, (Mouse) P58252
Entrez Gene ID: (Human) 1938, (Mouse) 13629
hematopoietic progenitor cell differentiation
response to ischemia
skeletal muscle contraction
translation
translational elongation
response to xenobiotic stimulus
glial cell proliferation
response to estradiol
response to endoplasmic reticulum stress
skeletal muscle cell differentiation
response to hydrogen peroxide
response to ethanol
positive regulation of translation
response to folic acid
translation at postsynapse
cellular response to brain-derived neurotrophic factor stimulus
positive regulation of cytoplasmic translation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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