CaptureSelect™ Alexa Fluor™ 488 Anti-C-tag Conjugate consists of a 13 kDa recombinant single domain antibody fragment (VHH affinity ligand) with high affinity and selectivity for the 4-amino-acid 'C-tag' peptide tag E‐P‐E‐A (glutamic acid-proline-glutamic acid-alanine). The anti-C-tag affinity ligand recognizes the E-P-E-A peptide when this tag is fused directly to the C-terminus of a protein. The affinity ligand—constructed as a mono-specific bihead—is chemically conjugated to Alexa Fluor 488 via an appropriate spacer that retains the binding reactivity of the ligand.
Applications for CaptureSelect Alexa Fluor488 Anti-C-tag Conjugate include detection of C-tag fusion proteins or imaging and flow cytometry when the C-tag is expressed and displayed by cells.
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).