The GeneChip™ DNA Labeling Reagent is used with the Prokaryotic Target Labeling Assay for 3' expression studies with GeneChip prokaryotic Brand Arrays. The assay utilizes reverse transcriptase and random hexamer primers to produce DNA complementary to the RNA. The cDNA products are then fragmented by DNase I and labeled with terminal transferase and biotinylated GeneChip DNA Labeling Reagent at the 3' termini.

Functionally tested on GeneChip Brand Arrays, we recommend the Prokaryotic Target Labeling Assay and associated reagents for use in prokaryotic gene expression studies.

Prokaryotic Target Labeling Assay components include:
• Control Oligo B2, 3nM
• GeneChip DNA Labeling Reagent
• GeneChip Poly-A RNA Control Kit

Detailed instructions for using this reagent are described in Section 3, Chapter 1 of the GeneChip Expression Analysis Technical Manual.

Each kit contains 1 vial of 60 µL of the GeneChip DNA Labeling Reagent, 7.5 mM, sufficient for 30 labeling reactions.

Related Links
GeneChip™ Wash Buffer A