Applied Biosystems™

GeneChip™ Wash Buffer B

GeneChip™ Wash Buffer B is used with the Prokaryotic Target Labeling Assay for 3' expression studies with GeneChip prokaryotic Brand Arrays.

Catalog Number	Quantity
900722	600 mL

Catalog number 900722

Price (USD)

Quantity:

600 mL

GeneChip™ Wash Buffer B is used with the Prokaryotic Target Labeling Assay for 3' expression studies with GeneChip prokaryotic Brand Arrays. The assay utilizes reverse transcriptase and random hexamer primers to produce DNA complementary to the RNA. The cDNA products are then fragmented by DNase I and labeled with terminal transferase and biotinylated with GeneChip DNA Labeling Reagent at the 3' termini.

Functionally tested on GeneChip Brand Arrays, we recommend the Prokaryotic Target Labeling Assay and associated reagents for use in prokaryotic gene expression studies.

Prokaryotic Target Labeling Assay components include:

Control Oligo B2, 3nM
GeneChip DNA Labeling Reagent
GeneChip Poly-A RNA Control Kit

Related Links:

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)Transcriptome Profiling

Product LineGeneChip

Quantity600 mL

Reagent TypeWash Buffer

TypeWash Buffer B

Unit SizeEach

Frequently asked questions (FAQs)

What is the shelf life of this the DNA Labeling reagent (Cat. No. 900542)?

The DNA Labeling Reagent has about 12 months of dating from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Do the reagents in the GeneChip HT Hybridization, Wash, and Stain Kit (Cat. No. 901219) have the same formulation as the GeneChip Hybridization, Wash, and Stain Kit for cartridges (Cat. No. 900720)?

The GeneChip HT hybridization, Wash Stain Kit (Cat. No. 901219) is part of an older kit that is discontinued and no longer available.
The kit consisted of:
- HT Pre-Hyb mix, HT 1.2X Hyb mix, HT Stain Cocktail 1& 3, HT Stain Cocktail 2, HT Wash Buffers A, HT Wash Buffer B, and HT Array Holding Buffer.

The GeneChip HWS kit (Cat. No. 900720) is a current product that can be ordered.
This kit consists of:
- Pre-Hyb module, 2X Hyb mix, DMSO, Nuclease Free Water, Stain Module (Stain Cocktail 1 and Stain Cocktail 2) Wash Buffers, Wash Buffer B, and Array Holding Buffer.

The HT kit was formulated for high throughput usage. Therefore, the formulations differ. The Wash buffers A and B are similar, but they have slight variations in terms of additives.
The Hyb mixes have different concentrations. The stain cocktails have different stain mixtures (Stain 1 & Stain 3 vs Stain 1).
Therefore, we do not recommend swapping any of these reagents.
Only the Array Holding Buffer is the same and could be swapped.
The exact components and concentrations of the reagents are proprietary information and thus cannot be disclosed.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.