As of April 2012, this product is the catalog replacement for Product No. 0820234DJ, a custom formulation that was available for web ordering. The formulation, raw materials, and manufacturing process are identical. For more information, please contact our Technical Service team.

MEM, high glucose, high sodium bicarbonate This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: A1451801

Gibco™  Related applications: Mammalian Cell Culture

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using our media selector tool.

This MEM is modified as follows:


WithWithout
• Phenol red• L-glutamine
• 6 g/L D-glucose• HEPES
• 3.7 g/L sodium bicarbonate 


Gibco® MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Product Intended Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® MEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Glutamine: No Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: No Sodium Pyruvate
Product Size: 500 mL
Volume (Metric): 500 ml
Serum Supplementation: Standard Serum Supplementation
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8 Deg;C. Protect from light



Shipping conditions: Ambient



Shelf life: 12 months from date of manufacture

Documents

Manuals & protocols

Recommended products