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Bethyl Laboratories
Pur-alpha Polyclonal Antibody
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FIGURE: 1 / 1
Pur-alpha Antibody (A303-543A-T) in WB
Detection of human and mouse Pur-alpha by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Pur-alpha antibody (Product # A303-543A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. Pur-alpha was more efficiently immunoprecipitated by rabbit anti-Pur-alpha antibody A303-544A, which recognizes a downstrea... View More
Product Details
A303-543A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1 and 50 of Human Purine-Rich Element Binding Protein Alpha.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Pur alpha; Purine-rich single-stranded DNA-binding protein alpha; Transcriptional activator protein Pur-alpha
Gene Aliases: CAGER-1; MRD31; NEDRIHF; PUR-ALPHA; PUR1; PURA; PURALPHA; ssCRE-BP
UniProt ID: (Human) Q00577, (Mouse) P42669
Entrez Gene ID: (Human) 5813, (Mouse) 19290
translation repressor activity, nucleic acid binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
double-stranded DNA binding
double-stranded telomeric DNA binding
single-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
RNA binding
protein binding
purine-rich negative regulatory element binding
SMAD binding
DNA-binding transcription factor binding
transcription regulator inhibitor activity
transcription factor binding
poly(A) RNA binding
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
DNA replication initiation
regulation of transcription from RNA polymerase II promoter
nervous system development
cell proliferation
positive regulation of cell proliferation
negative regulation of translation
negative regulation of transcription, DNA-templated
lymphocyte proliferation
epithelial cell proliferation
dendritic transport of messenger ribonucleoprotein complex
DNA unwinding involved in DNA replication
transcription, DNA-templated
regulation of transcription, DNA-templated
apoptotic process
mitotic cell cycle checkpoint
cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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