Thermo Scientific™

LabCoat Live™ Training Course for Real-Time PCR (qPCR)

Catalog number:  A30851

 Related applications:

Real Time PCR (qPCR)

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LabCoat Live: Introduction to Gene Expression Analysis using the ΔΔCt Method, 1 week
Learn the fundamentals of how to perform gene expression using qPCR and the relative quantification (RQ or ΔΔCt) method of analysis. This course provides professional, interactive education via online lecture(s) and a self-paced hands-on lab experiment using provided protocol(s) and reagents. At the conclusion of this course, participants should be able to:

• Explain gene expression using qPCR, including experimental design choices and important validation steps to help ensure accurate results
• Perform a basic gene expression experiment
• Determine whether to use the RQ (ΔΔCt ) method or the Relative Standard Curve method for data analysis
• Analyze data using the RQ (ΔΔCt) method

Tuition includes*:
• Two 1-hour interactive online lectures
• 1 month of access to class webpage, including downloadable notes and protocol, support videos, and question and answer forum
• A training kit that includes reagents needed to perform the course protocol

*The participant is responsible for providing an Applied Biosystems real-time PCR instrument (7900HT Fast, QuantStudio series, 7500 series, StepOne/StepOnePlus, or ViiA 7 Real-Time PCR System) and materials necessary to set up a qPCR experiment (i.e., optical plates and adhesive covers, aerosol-resistant filter tips, pipettes, gloves, racks, vortex, microfuge tubes). In addition, participants will need a computer with internet access and minimum system requirements.

For more detailed course information including dates and locations, visit application and instrument training courses.

For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): 7500 System, 7900HT System, QuantStudio™, StepOne™ System, StepOnePlus™ System, ViiA™ 7 System
Plan Duration: 1 Week Training
Product Line: LabCoat Live™
Product Size: 1 class, Intro to Gene Expr Anlys, 1 week
Shipping Condition: Approved for shipment on Wet or Dry Ice
Type: Other Accessories

Contents & storage

• Reagent experiment kit
• One-month access to the message board
• Lecture notes
• Experiment setup videos


Manuals & protocols