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Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor™ 488-10 nm colloidal gold
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FIGURE: 1 / 1
Mouse IgG (H+L) Secondary Antibody (A-31561) in ICC/IF
Immunofluorescence analysis of Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor 488-10 nm colloidal gold was performed using MCF-7 cells stained with Cytokeratin 19 (RCK108) Mouse Monoclonal Primary Antibody (Product # MA5-12613). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primar... View More
Product Details
A-31561
Applications
Tested Dilution
Immunocytochemistry (ICC/IF)
1 µg/mL
Immunomicroscopy (IM)
0.2-10 µg/mL
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobin
Conjugate
Alexa Fluor™ 488-10 nm colloidal gold
Alexa Fluor™ 488-10 nm colloidal gold
Alexa Fluor™ 488-10 nm colloidal gold
Form
Liquid
Concentration
30 µg/mL
Amount
15 µg
Purification
purified
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
This 10 nm colloidal gold conjugate may be used as a probe in immunoblotting, light microscopy, fluorescence microscopy or electron microscopy. The fluorescence of this conjugate can be easily detected by standard techniques, but the ease of visualization of colloidal gold can be greatly improved using silver-enhancement methods, such as those we provide in the LI Silver Enhancement Kit (Cat. no. L-24919).
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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