Applied Biosystems™

MicroSEQ™ ID Purification Combo Kit v2.0, with cartridges

Catalog number: A37741
Applied Biosystems™

MicroSEQ™ ID Purification Combo Kit v2.0, with cartridges

Catalog number: A37741
Catalog Number
A37741
Unit Size
1 kit
Price (USD)
Price: 223.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A377411 kit
Price: 223.00
Your Price:
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Product Overview
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The MicroSEQ ID Purification Combo Kit v2.0 includes reagents for post-PCR and post-sequencing clean-up in the MicroSEQ ID microbial identification workflow. This version contains one tube of ExoSAP-IT Express Reagent for post-PCR clean-up and MicroSEQ ID Sequencing Clean-up Cartridges for post-sequencing clean-up.

ExoSAP-IT Express Reagent removes unincorporated dNTPs and primers from the post-amplification PCR mix in 5 minutes compared to 30 minutes with the original ExoSAP-IT Reagent while producing equivalent results. The MicroSEQ ID Sequencing Clean-up Cartridges provide a convenient and simple way to remove dye terminators, dNTPs, salts, and residual primers from cycle sequencing reactions. The end products can be used directly for sequencing on any Applied Biosystems genetic analyzer.

Comparability studies demonstrated equivalent results between the original ExoSAP-IT Reagent and the improved ExoSAP-IT Express Reagent coupled with the MicroSEQ ID Sequencing Clean-Up kit in the MicroSEQ ID workflow (see figure 1). Both ExoSAP-IT and ExoSAP-IT Express enzyme-treated PCR samples produced very similar sequencing results and the correct identification result.

ExoSAP-IT Express Reagent can be used downstream of the following PCR amplification kits:
• MicroSEQ 500 16S rDNA PCR Kit
• MicroSEQ Full Gene 16S rDNA PCR Kit
• MicroSEQD2 rDNA Fungal PCR Kit

MicroSEQ ID Sequencing Clean-up Cartridges can be used downstream of the following sequencing kits:
• MicroSEQ 500 16S rDNA Sequencing Kit
• MicroSEQ Full Gene 16S rDNA Sequencing Kit
• MicroSEQ D2 rDNA Fungal Sequencing Kit

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Cartridge
For Use With (Application)
Sequencing
High-throughput Compatibility
Manual Protocols
No. of Reactions
100 Reactions
Product Line
MicroSEQ™
Shipping Condition
Approved for shipment on Wet or Dry Ice

Contents & Storage

• ExoSAP-IT Express (100 reactions ), store at -5 to -30°C
• MicroSEQ ID Sequencing Clean-up Cartridges (36 cartridges), store at 2–8°C

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