The Applied Biosystems TrueMark MSI Assay is a low-input, single-amplification solution for the rapid identification of microsatellite instability (MSI) in formalin-fixed paraffin-embedded (FFPE) samples from multiple tumor tissue types for research applications. The TrueMark MSI Assay leverages an expanded 6-dye set to incorporate additional markers and does not require the use of a tumor-normal match, thereby cutting in half the number of wells required for each sample, as well as reducing the sample handling burden. The assay is compatible with the Applied Biosystems SeqStudio and 3500 Series genetic analyzers.
MSI analysis software The associated TrueMark MSI Analysis Software (included at no additional charge) is designed specifically for the TrueMark MSI Assay to enable automated calling of markers and samples, offering greater ease of analysis by reducing the manual QC, analysis, and review required to determine sample status. The automated calling functions without the use of a tumor-normal match, but it is also compatible with tumor-normal pairs.
The TrueMark MSI Assay features include: • Expanded coverage—13 MSI markers --BAT-25, BAT-26, BAT-40, CAT-25, NR-21, NR-22, NR-24, NR-27 (publicly known MSI markers) --ABI-16, ABI-17, ABI-19, ABI-20A, ABI-20B (MSI markers proprietary to Thermo Fisher Scientific) --PentaD, TH-01 (sample ID markers) • Optional normal control—no requirement for use of tumor-normal match for interpretation • Low input—use as little as 2 ng FFPE-derived DNA • Automated calling—analysis software includes automated calling of markers and samples to accelerate initial data interpretation • Simple analysis software—streamline QC, analysis, and review of samples
Analysis software for download • TrueMark MSI Analysis Software
Supplied reagents • TrueMark MSI Primer mix • TrueMark MSI Master mix • TrueMark MSI No Template Control • TrueMark MSI Amplification Control