Applied Biosystems™

TrueMark™ MSI Assay

Catalog number:  A45295

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Description

The Applied Biosystems TrueMark MSI Assay is a low-input, single-amplification solution for the rapid identification of microsatellite instability (MSI) in formalin-fixed paraffin-embedded (FFPE) samples from multiple tumor tissue types for research applications. The TrueMark MSI Assay leverages an expanded 6-dye set to incorporate additional markers and does not require the use of a tumor-normal match, thereby cutting in half the number of wells required for each sample, as well as reducing the sample handling burden. The assay is compatible with the Applied Biosystems SeqStudio and 3500 Series genetic analyzers.

MSI analysis software
The associated TrueMark MSI Analysis Software (included at no additional charge) is designed specifically for the TrueMark MSI Assay to enable automated calling of markers and samples, offering greater ease of analysis by reducing the manual QC, analysis, and review required to determine sample status. The automated calling functions without the use of a tumor-normal match, but it is also compatible with tumor-normal pairs.

The TrueMark MSI Assay features include:
Expanded coverage—13 MSI markers
    --BAT-25, BAT-26, BAT-40, CAT-25, NR-21, NR-22, NR-24, NR-27 (publicly known MSI markers)
    --ABI-16, ABI-17, ABI-19, ABI-20A, ABI-20B (MSI markers proprietary to Thermo Fisher Scientific)
    --PentaD, TH-01 (sample ID markers)
Optional normal control—no requirement for use of tumor-normal match for interpretation
Low input—use as little as 2 ng FFPE-derived DNA
Automated calling—analysis software includes automated calling of markers and samples to accelerate initial data interpretation
Simple analysis software—streamline QC, analysis, and review of samples

Analysis software for download
• TrueMark MSI Analysis Software

Supplied reagents
• TrueMark MSI Primer mix
• TrueMark MSI Master mix
• TrueMark MSI No Template Control
• TrueMark MSI Amplification Control

Required reagents not supplied in the TrueMark MSI Assay
GeneScan 600 LIZ Size Standard v2.0
Hi-Di Formamide
DS-36 Matrix Standard (Dye Set J6)

The TrueMark MSI Assay has been tested with RecoverAll Total Nucleic Acid Isolation Kit for FFPE and Qiagen QIAamp™ DSP DNA FFPE Tissue Kit for sample preparation. Please note these are not included in the kit.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: TrueMark™
Product Size: 100 reactions
Shipping Condition: Dry Ice
Test Type: Oncology

Contents & storage

• TrueMark MSI Assay Master Mix
• TrueMark MSI Assay Primer Mix
• TrueMark MSI Assay Amplification Control
• TrueMark MSI Assay No-Template Control

Store at -15 to -25°C, then 2–8°C after first use.