Invitrogen™

MultiShot™ FlexPlate Stbl3™ Competent Cells

Catalog number:  C7381201

Related applications:

Transformation

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Description

MultiShot FlexPlate Stbl3 chemically competent E. coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 wells to increase productivity for medium-throughput bacterial transformations. MultiShot FlexPlate Stbl3 competent E. coli cells are designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli, these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower Lentiviral Expression vectors and other retroviral vectors. The DNA yield from Stbl3 cells is often >10-fold higher than alternative strains such as TOP10 (see table).

Reduce unwanted recombination with Stbl3 cells
MultiShot FlexPlate Stbl3 Competent Cells are derived from the HB101 E. coli strain and are recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats (e.g., ViraPower Lentiviral Expression kits). The transformation efficiency of these cells is greater than 1 × 107 cfu/µg plasmid DNA.

Key features of the MultiShot FlexPlate Stbl3 Competent Cells include:
Optimal performance for the propagation of lentiviral vectors
mcrB, mrr for enhanced genomic cloning capacity
recA13 for reduced occurrence of non-specific recombination in cloned DNA
High DNA yields

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be transformed efficiently by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
Increase throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F-mcrB mrr hsdS20(rB-, mB-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ- leu mtl-1

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: Stbl3™
Blue/White Screening: No
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Lacks F' Episome
High-throughput Compatibility: High-throughput Compatible
Plasmid: May be used for plasmids > 20 kb
Product Line: MultiShot™, Stbl3™
Propagating ccdB Vectors: Not for ccdB vector propagation
Shipping Condition: Dry Ice
Format: 96-well plate
Species: E. coli

Contents & storage

• MultiShot FlexPlate Stbl3 Competent Cells: One 96-well plate, 20 µL per well, store at –80°C
• pUC19 DNA (10 pg/µL): 1 vial, 50 µL, store at –20°C
• S.O.C. medium: 2 bottles, 10 mL each, store at 4°C or room temperature