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OriGene
NEDD4 Monoclonal Antibody (OTI6E12), TrueMAB™
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FIGURE: 1 / 1
NEDD4 Antibody (CF812646) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEDD4 (RC212738, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NEDD4. Positive lysates LY405051 (100 µg) and LC405051 (20 µg) can be purchased separately from OriGene.
Product Details
CF812646
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI6E12
Immunogen
Human recombinant protein fragment corresponding to amino acids 160-460 of human NEDD4 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; KIAA0093; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase; OTTHUMP00000217840; OTTHUMP00000217841; OTTHUMP00000217842; PIG53; receptor-potentiating factor 1; unnamed protein product
Gene Aliases: KIAA0093; NEDD4; NEDD4-1; PIG53; RPF1
UniProt ID: (Human) P46934
Entrez Gene ID: (Human) 4734
ubiquitin-protein transferase activity
protein binding
channel inhibitor activity
transferase activity
potassium channel inhibitor activity
sodium channel inhibitor activity
enzyme binding
protein domain specific binding
beta-2 adrenergic receptor binding
ionotropic glutamate receptor binding
ubiquitin binding
ion channel binding
phosphoserine binding
phosphothreonine binding
ubiquitin protein ligase activity
RNA polymerase binding
proline-rich region binding
negative regulation of transcription from RNA polymerase II promoter
adaptive immune response
outflow tract morphogenesis
endocardial cushion development
transcription from RNA polymerase II promoter
ubiquitin-dependent protein catabolic process
protein monoubiquitination
protein targeting to lysosome
sodium ion transport
immune response
cellular response to DNA damage stimulus
lysosomal transport
nervous system development
neuromuscular junction development
regulation of cell communication
negative regulation of sodium ion transport
regulation of macroautophagy
protein ubiquitination
regulation of signaling
negative regulation of vascular endothelial growth factor receptor signaling pathway
neuron projection development
receptor internalization
receptor catabolic process
cellular response to UV
T cell activation
regulation of protein catabolic process
regulation of membrane potential
glucocorticoid receptor signaling pathway
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
development involved in symbiotic interaction
innate immune response
positive regulation of protein catabolic process
viral budding
positive regulation of nucleocytoplasmic transport
blood vessel morphogenesis
regulation of dendrite morphogenesis
regulation of synapse organization
progesterone receptor signaling pathway
response to calcium ion
establishment of localization in cell
positive regulation of protein kinase B signaling
regulation of biological quality
protein K63-linked ubiquitination
negative regulation of potassium ion export across plasma membrane
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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