I am planning to use the EnzChek Caspase-3 Assay Kit #1 with Z-DEVD-AMC substrate. What are the fluorescence excitation/emission maxima of the cleaved substrate?
The basis for the EnzChek Caspase-3 Assay is the 7-amino-4-methylcoumarin-derived substrate Z-DEVD-AMC (where Z represents a benzyloxycarbonyl group),
which is weakly fluorescent in the UV range (excitation/emission ~330/390 nm), but yields a bright blue fluorescent product (excitation/emission ~342/441 nm) upon proteolytic cleavage. Measure the fluorescence (excitation/emission ~342/441 nm) using appropriate excitation and emission filters or settings.
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