Thermo Scientific T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.
The minimal substrate is a nucleoside 3',5'-biphosphate in intermolecular reaction and oligonucleotide of 8bases in intramolecular reaction.
• RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate • Joining RNA to RNA • Synthesis of oligoribonucleotides and oligodeoxyribonucleotides • Specific modifications of tRNAs • Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5' RACE (Rapid Amplification of cDNA Ends) • Site-specific generation of composite primers for PCR
The recommended BSA concentration in the reaction mixture is 0.1mg/mL.
For Research Use Only. Not for use in diagnostic procedures.