Thermo Scientific™

T4 RNA Ligase (10 U/µL)

Catalog number:  EL0021

 Related applications:

Restriction Enzyme Cloning

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Thermo Scientific T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.

The minimal substrate is a nucleoside 3',5'-biphosphate in intermolecular reaction and oligonucleotide of 8bases in intramolecular reaction.


• RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate
• Joining RNA to RNA
• Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
• Specific modifications of tRNAs
• Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5' RACE (Rapid Amplification of cDNA Ends)
• Site-specific generation of composite primers for PCR


The recommended BSA concentration in the reaction mixture is 0.1mg/mL.
For Research Use Only. Not for use in diagnostic procedures.


Enzyme: T4 RNA Ligase
Product Size: 1,000 units


Manuals & protocols