FISH Tag™ DNA Kits with Alexa Fluor™ Dyes

Choose from three, single-color FISH Tag DNA kits with Alexa Fluor dye options (green, orange, or red). The FISH DNA multicolor kit contains four spectrally distinct dyes (green, orange, red, and deep-red) to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and in situ analysis of mitochondrial DNA localization and content. All kits include 16 reagents and come with a comprehensive, detailed protocol to help ensure your success.

Complete workflow solution for FISH

FISH Tag kits employ a two-step labeling technology: nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor dyes. PureLink nucleic acid purification technology is used for fast and efficient purification of the labeled probe and SlowFade Gold Antifade Mountant preserves the fluorescent signal during imaging.

DNA targets expressed in a tissue or genes present on a chromosome can be localized using FISH Tag technology. Detection of a nucleic acid target in situ is achieved through hybridization of a complementary sequence with a fluorescent dye–labeled nucleic acid probe to the specimen. Once the hybridization assay is complete, the specimen is viewed under a fluorescence microscope to visualize the hybridized fluorescent probe. The dyes available in the FISH Tag DNA kits are compatible with standard filter sets found on most fluorescence microscopes. Alexa Fluor dyes are brighter and more photostable than traditional fluorescent labels, providing higher resolution and improved signal-to-noise ratios compared to conventional dyes.

See the user manual for information regarding how to calculate base:dye ratio and nucleic acid concentration.