Anza™ T4 DNA Ligase Master Mix

Catalog number:  IVGN2104

 Related applications:

Restriction Enzyme Cloning

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The Invitrogen Anza™ T4 DNA Ligase Master Mix facilitates the joining of abutted 5’-phosphate and 3’-hydroxyl termini in duplex DNA through the formation of a phosphodiester bond. It can be used to join DNA fragments with both sticky ends and blunt ends, and to repair nicks in double-stranded DNA with 3'-hydroxyl and 5'-phosphate ends. Anza T4 DNA Ligase is formulated as a 4X concentrated master mix. Ligation can be performed with DNA in water, TE, elution buffer, or 1X Anza™ buffers.

• Ligation complete in 15 minutes at room temperature
• A single ligase master mix for both sticky-end and blunt-end ligation reactions
• Ready-to-use master mix format reduces pipetting steps
• 4X concentration enables more dilute DNA to be used in ligation reaction without concentrating

Anza T4 DNA Ligase Master Mix is part of the Anza™ Restriction Enzyme Cloning System, unifying the traditional cloning processes. Other Anza DNA Modifying Enzymes include the Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit.
For Research Use Only. Not for use in diagnostic procedures.


Enzyme: T4 DNA Ligase
Product Line: Anza™
Product Size: 50 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

250 µL Anza T4 DNA Ligase Master Mix

Store at -5 to -30°C.


Manuals & protocols