The Invitrogen™ Anza™ T4 PNK (polynucleotide kinase) Kit is used to perform 5’-phosphorylation of DNA and oligonucleotides. T4 PNK exhibits 5’ polynucleotide kinase and 3’ phosphatase activity, catalyzing the transfer of the terminal phosphate of ATP to a 5’ hydroxyl group of a nucleic acid.
When preparing DNA for the ligation step in cloning, either the insert DNA or the vector DNA should contain a 5’ phosphate. Often PCR-amplified DNA is treated with T4 PNK to phosphorylate the 5’ end for subsequent cloning ligation.Benefits:
• Fast 15-minute incubation at room temperature
• Phosphorylaton can be performed with DNA or oligonucleotides in water, TE, or 1X Anza buffers
The Anza T4 PNK Kit is part of the Anza™ Restriction Enzyme Cloning System, unifying the traditional cloning processes. Other Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix
, Anza DNA Blunt End Kit
, and Anza DNA End Repair Kit
For Research Use Only. Not for use in diagnostic procedures.