The Invitrogen Anza™ T4 DNA Ligase Master Mix facilitates the joining of abutted 5’-phosphate and 3’-hydroxyl termini in duplex DNA through the formation of a phosphodiester bond. It can be used to join DNA fragments with both sticky ends and blunt ends, and to repair nicks in double-stranded DNA with 3'-hydroxyl and 5'-phosphate ends. Anza T4 DNA Ligase is formulated as a 4X concentrated master mix. Ligation can be performed with DNA in water, TE, elution buffer, or 1X Anza™ buffers.
Benefits: • Ligation complete in 15 minutes at room temperature • A single ligase master mix for both sticky-end and blunt-end ligation reactions • Ready-to-use master mix format reduces pipetting steps • 4X concentration enables more dilute DNA to be used in ligation reaction without concentrating