Anza™ DNA End Repair Kit

Catalog number: IVGN2504

Invitrogen™  Related applications: Restriction Enzyme Cloning

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Description

The Invitrogen™ Anza™ DNA End Repair Kit is used to convert DNA with cohesive ends to blunt-ended 5’-phosphorylated DNA for use in blunt-end ligation reactions. The included pre-mixed Anza™ DNA End Repair Mix contains T4 DNA polymerase, Klenow Fragment, and T4 PNK (polynucleotide kinase) to minimize pipetting, while the Anza™ 10X End Repair Buffer contains ATP and dNTPs to facilitate the activity of the enzyme mix.

Benefits:
Convenient—pre-mixed enzyme mixture for superior convenience and reduction of pipetting
Efficient—simultaneous blunting and phosphorylation
Compatible—use directly in Anza restriction enzyme digestion reaction mixture following heat inactivation
Fast—end repair complete in 15 minutes

The Anza DNA End Repair Kit is part of the Anza™ Restriction Enzyme Cloning System, unifying the traditional cloning processes. Other Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza T4 PNK Kit, and Anza DNA Blunt End Kit.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme: Klenow Fragment, T4 DNA polymerase, T4 Polynucleotide Kinase
Product Size: 20 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

100 µL Anza DNA End Repair Mix
200 µL Anza 10X End Repair Buffer

Store at -5 to -30°C.

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Manuals & protocols

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