The Invitrogen™ Anza™ DNA End Repair Kit is used to convert DNA with cohesive ends to blunt-ended 5’-phosphorylated DNA for use in blunt-end ligation reactions. The included pre-mixed Anza™ DNA End Repair Mix contains T4 DNA polymerase, Klenow Fragment, and T4 PNK (polynucleotide kinase) to minimize pipetting, while the Anza™ 10X End Repair Buffer contains ATP and dNTPs to facilitate the activity of the enzyme mix.
Benefits: • Convenient—pre-mixed enzyme mixture for superior convenience and reduction of pipetting • Efficient—simultaneous blunting and phosphorylation • Compatible—use directly in Anza restriction enzyme digestion reaction mixture following heat inactivation • Fast—end repair complete in 15 minutes