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PCR Master Mix (2X)Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
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| Catalog Number | No. of Reactions |
|---|---|
| K0171 | 200 Reactions |
| K0172 | 1000 Reactions |
Catalog number K0171
Price (USD)/ 200 reactions
155.65
Online exclusive
180.00 Save 24.35 (14%)
-
No. of Reactions:
200 Reactions
Price (USD)/ 200 reactions
155.65
Online exclusive
180.00 Save 24.35 (14%)
PCR Master Mix (2X)
Catalog numberK0171
Price (USD)/ 200 reactions
155.65
Online exclusive
180.00 Save 24.35 (14%)
-
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
- Convenient, ready-to-use mix
- Thermostable—half life is more than 40 min. at 95°C
- Generates PCR products with 3'-dA overhangs
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
- Routine PCR amplification of DNA fragments up to 5 kb
- High throughput PCR
- DNA labeling
Note
The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceLow
PolymeraseTaq DNA Polymerase
Reaction SpeedStandard
Product TypePCR Master Mix
For Use With (Application)Standard PCR
Concentration2X
Fidelity (vs. Taq)1X
Hot StartNo
No. of Reactions200 Reactions
Overhang3'-A
Reaction FormatSuperMix or Master Mix
Starting MaterialDNA
Unit Size200 reactions
Contents & Storage
• 2X PCR Master Mix, 4 x 1.25 mL
• Nuclease-free water, 4 x 1.25 mL
Store at -20°C.
• Nuclease-free water, 4 x 1.25 mL
Store at -20°C.