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Thermo Scientific™

Maxima™ H Minus cDNA Synthesis Master Mix

Catalog number: M1681
Thermo Scientific™

Maxima™ H Minus cDNA Synthesis Master Mix

Catalog number: M1681
Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix, available with or without dsDNase, provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications and reproducible cDNA synthesis at elevated temperatures (50–65°C) within 30 minutes.
 
Catalog Number
M1681
Unit Size
Each
Includes
Kit with dsDNase
No. of Reactions
50 Reactions
Price (USD)
Price: 474.00
Your price:
Availability
-
Full specifications
Quantity
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseMaxima H Minus
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit with dsDNase
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit only
No. of Reactions
50 Reactions
Price (USD)
Price: 407.00
Online price: 350.65
Your price:
Availability
-
Full specifications
Quantity
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseMaxima H Minus
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit only
No. of Reactions
200 Reactions
Price (USD)
Price: 1,276.00
Online price: 1,108.65
Your price:
Availability
-
Full specifications
Quantity
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseMaxima H Minus
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit with dsDNase
No. of Reactions
200 Reactions
Price (USD)
Price: 1,526.00
Your price:
Availability
-
Full specifications
Quantity
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseMaxima H Minus
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit with dsDNase
Unit SizeEach
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeIncludesNo. of ReactionsPrice (USD)AvailabilityQuantity
M1681Full specifications
Each Kit with dsDNase50 Reactions
Price: 474.00
Your price:
-
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseMaxima H Minus
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit with dsDNase
Unit SizeEach
M1661Full specifications
Each Kit only50 Reactions
Price: 407.00
Online price: 350.65
Your price:
-
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseMaxima H Minus
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
M1662Full specifications
Each Kit only200 Reactions
Price: 1,276.00
Online price: 1,108.65
Your price:
-
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseMaxima H Minus
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
M1682Full specifications
Each Kit with dsDNase200 Reactions
Price: 1,526.00
Your price:
-
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseMaxima H Minus
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit with dsDNase
Unit SizeEach
Showing 4 of 4

The master mix contains Maxima H Minus Reverse Transcriptase (RT) and RiboLock RNase Inhibitor. Maxima H Minus RT is an advanced enzyme derived from M-MuLV RT by in vitro evolution. The enzyme features high thermostability, robustness, processivity, increased cDNA synthesis rate, and lack of RNase H activity. The recombinant RiboLock RNase Inhibitor effectively protects RNA template from degradation by RNases A, B, and C at temperatures up to 55°C. The master mix also contains reaction buffer, dNTPs, oligo (dT)18, and random hexamer primers. A separate tube of No-RT control mix is also provided for convienient RT negative control.

Both kits are capable of reproducible cDNA synthesis at elevated temperatures (50°C–65°C). The synthesis reaction is typically complete in 15–30 minutes. The included double-strand specific DNase in the Maxima H Minus cDNA Synthesis Master Mix with dsDNase allows removal of genomic DNA from RNA samples in two minutes without affecting the quality or quantity of RNA. This dsDNase is also available separately (Cat. No. EN0771).

Features of Maxima H Minus cDNA Synthesis Master Mix include:
• One-tube master mix helps reduce pipetting steps and enhanced consistency in RT-qPCR results
• Increased RT efficiency across a wide range of input RNA amounts and gene targets
• High thermostability to allow RT reactions at 50–65°C temperature range
• Integrated step of genomic DNA removal from RNA samples (Maxima H Minus cDNA Synthesis Master Mix with dsDNase only)

Additional information about reaction components
• The No RT control mix contains all the components in the Maxima H Minus cDNA Synthesis Master Mix except RT. The presence of a PCR product in the No RT control reaction indicates that the reaction is contaminated with DNA. To further enhance genomic DNA elimination efficiency, template RNA incubation with dsDNase (Cat. No. EN0771) is strongly recommended.
• Nuclease-free water is provided for reaction setup and dilution of sample DNA. The absence of exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.
• To further enhance genomic DNA elimination efficiency, template RNA incubation with the included dsDNase is strongly recommended. (Maxima H Minus cDNA Synthesis Master Mix with dsDNase only)
• The dsDNase is used for rapid and safe removal of contaminating genomic DNA from RNA samples. dsDNase is easily inactivated by moderate heat treatment (55°C). It allows for a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. (Maxima H Minus cDNA Synthesis Master Mix with dsDNase only)

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

• Maxima H Minus cDNA Synthesis Master Mix (1 x 200 μL)
• Maxima H Minus cDNA Synthesis Master Mix 'No RT' Control (1 x 200 μL)
• dsDNase (1 x 50 μL)
• 10X dsDNase buffer (1 x 100 μL)
• Nuclease-free water (1 x 1.25 mL)

Store at –5 to –30°C.

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