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Invitrogen
PAX6 Monoclonal Antibody (13B10-1A10)
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FIGURE: 1 / 19
PAX6 Antibody (MA1-109) in ICC/IF
Immunofluorescent analysis of PAX6 (green) in human B3 lens epithelial cells (left panel) and negative control U-2 OS cells (right panel). The cells were fixed with formaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 1% Blocker BSA in PBS (Product # 37525) for 15 minutes, all at room temperature. Cells were stained with a PAX6 monoclonal antibody (Product # MA1-109) in 1% Blocker BSA in PBS (Product # 37525) at a dilution of 1:100 for 1 hour at room temperature, and then incubated with ... View More
Product Details
MA1-109
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
13B10-1A10
Immunogen
Purified internal fragment of human recombinant PAX6 expressed in E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Western blot analysis of MA1-109 detects an ~47 kDa protein in whole cell lysates from early passage human B3 lens epithelial cells and in mouse eye lysate. An additional unknown band at ~36 kDa is also detected in B3 cell lysates. This antibody will detect additional nonspecific bands in lysates with very low basal PAX6 expression. Specificity of the antibody was confirmed in B3 and mouse eye lysates (positive controls) compared to U-2 OS human osteosarcoma and NIH/3T3 mouse embryonic fibroblasts (negative controls).
MA1-109- can be used for immunofluoresence and flow cytometry analysis of Pax6 in human neural stem cells.
Target Information
This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alternative protein PAX6; Aniridia; Aniridia 1; Aniridia 2; Aniridia type II protein; Dickie's small eye; Keratitis; MGC17209; Oculorhombin; paired box 6 PAX6-12a isoform; paired box 6 PAX6A-ASdel6 isoform; paired box 6 PAX6A-ASdel6-AS7 isoform; paired box 6 PAX6A-del6 isoform; paired box 6 PAX6B-ASdel6 isoform; paired box 6 PAX6D isoform; paired box gene 6; Paired Box Gene-6; paired box homeotic gene-6; Paired box protein Pax-6; Pax6(S); encoded by transcript variant 4; small eye; unnamed protein product
Gene Aliases: 1500038E17Rik; AEY11; AN; AN1; AN2; ASGD5; D11S812E; Dey; FVH1; Gsfaey11; MGDA; Pax-6; PAX6; Sey; WAGR
UniProt ID: (Human) P26367, (Mouse) P63015
Entrez Gene ID: (Human) 5080, (Mouse) 18508
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA binding
protein binding
protein kinase binding
chromatin DNA binding
ubiquitin protein ligase binding
histone acetyltransferase binding
sequence-specific DNA binding
co-SMAD binding
R-SMAD binding
HMG box domain binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
AT DNA binding
double-stranded DNA binding
transcription factor binding
transcription regulatory region DNA binding
negative regulation of transcription from RNA polymerase II promoter
establishment of mitotic spindle orientation
blood vessel development
eye development
cell fate determination
neuron migration
positive regulation of neuroblast proliferation
lens development in camera-type eye
regionalization
type B pancreatic cell differentiation
pancreatic A cell development
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
smoothened signaling pathway
nervous system development
neuroblast proliferation
negative regulation of neuroblast proliferation
axonogenesis
axon guidance
central nervous system development
brain development
sensory organ development
salivary gland morphogenesis
visual perception
cell proliferation
response to wounding
anatomical structure morphogenesis
regulation of asymmetric cell division
organ morphogenesis
dorsal/ventral axis specification
anterior/posterior pattern specification
dorsal/ventral pattern formation
gene expression
regulation of gene expression
positive regulation of gene expression
ventral spinal cord development
pallium development
oligodendrocyte cell fate specification
cerebral cortex regionalization
forebrain dorsal/ventral pattern formation
commitment of neuronal cell to specific neuron type in forebrain
forebrain-midbrain boundary formation
telencephalon regionalization
pituitary gland development
habenula development
cerebral cortex development
signal transduction involved in regulation of gene expression
cell differentiation
neuron differentiation
keratinocyte differentiation
regulation of cell migration
epithelial cell differentiation
positive regulation of epithelial cell differentiation
forebrain development
lacrimal gland development
protein localization to organelle
eye photoreceptor cell development
glucose homeostasis
camera-type eye development
cell fate commitment
regulation of neuron differentiation
negative regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of timing of cell differentiation
embryonic camera-type eye morphogenesis
neuron fate commitment
astrocyte differentiation
negative regulation of epithelial cell proliferation
regulation of neurogenesis
negative regulation of neurogenesis
neurological system process
retina development in camera-type eye
iris morphogenesis
cornea development in camera-type eye
neural precursor cell proliferation
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
cellular response to leukemia inhibitory factor
negative regulation of neural precursor cell proliferation
neural crest cell migration
negative regulation of protein phosphorylation
epithelial cell development
pancreatic A cell differentiation
transcription, DNA-templated
multicellular organism development
negative regulation of cell proliferation
regulation of neuron projection development
rhombomere morphogenesis
olfactory bulb development
forebrain anterior/posterior pattern specification
regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification
regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification
regulation of transcription from RNA polymerase II promoter involved in somatic motor neuron fate commitment
interkinetic nuclear migration
hindbrain development
positive regulation of cell fate specification
positive regulation of glucagon secretion
insulin metabolic process
positive regulation of neuron migration
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