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Invitrogen
CD69 Monoclonal Antibody (H1.2F3)
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FIGURE: 1 / 5
CD69 Antibody (MA1-207) in ICC/IF
Immunofluorescent analysis of CD69 (green) in PHA-M (2% v/v, Product # 10576-015) treated Raji cells. Cells were blocked with blocking buffer (Product # 37525) for 30 min at room temparture and then stained with CD69 hamster monoclonal antibody (Product # MA1-207) at a dilution of 1:100 for 1 hour on ice. After washing with ice-cold PBS for 3 times, the cells were then stained with DyLight™ 488 Goat anti-hamster (Armenian) IgG antibody for 1 hour on ice. The cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature. Nucl... View More
Product Details
MA1-207
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
H1.2F3
Immunogen
Mouse dendritic epidermal T cell line Y245
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-207 detects CD69 from human samples.
MA1-207 has been successfully tested in western blot, immunofluorescence and flow cytometry applications.
In western blot, MA1-207 detects CXCR1/IL8RA (human) at approximate molecular weight of 22.5 kDa.
Target Information
CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activation inducer molecule; activation inducer molecule (AIM/CD69); AIM; BL-AC/P26; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; CD69; CD69 antigen (p60, early T-cell activation antigen); EA1; Early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; unnamed protein product; Very Early Activation Antigen
Gene Aliases: 5830438K24Rik; AI452015; AIM; BL-AC/P26; CD69; CLEC2C; EA1; GP32/28; MLR-3; VEA
UniProt ID: (Human) Q07108, (Mouse) P37217
Entrez Gene ID: (Human) 969, (Mouse) 12515
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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