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Invitrogen
HSPA9 Monoclonal Antibody (30A5)
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FIGURE: 1 / 7
HSPA9 Antibody (MA1-91639) in ICC/IF
Immunofluorescence analysis of HSPA9 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSPA9 Monoclonal Antibody (30A5) (Product # MA1-91639) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More
Product Details
MA1-91639
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Sheep,
Pig,
Rabbit,
Rat,
Xenopus
Published species
Chicken,
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
30A5
Immunogen
Grp75 isolated from human Molt4 cells.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
25 µg
Purification
Ammonium sulfate precipitation
Storage buffer
PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Western blot detects a band ~75 kDa. A suggested positive control is HeLa cell lysate (heat shocked).
Target Information
The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles. GRP75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP75 and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 70 kDa heat shock protein; 75 kDa glucose-regulated protein; C3H-specific antigen; catecholamine-regulated protein 40; CRP40; epididymis secretory sperm binding protein Li 124m; GRP 75; GRP-75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9A; heat shock 70kDa protein 9B (mortalin-2); Heat shock protein; heat shock protein 9; heat shock protein 9A; heat shock protein cognate 74; Heat shock protein family A member 9; heat shock protein, 74 kDa, A; heat shock protein, A; HSP; MGC4500; Mortalin; mortalin, perinuclear; mortalin-2; mortalin2; MOT; mtHSP70; p66 MOT; p66-mortalin; PBP74; Peptide-binding protein 74; Stress-70 protein, mitochondrial; unnamed protein product
Gene Aliases: 74kDa; CRP40; CSA; EVPLS; GRP-75; GRP75; HEL-S-124m; Hsc74; Hsp74; Hsp74a; HSPA9; hspa9-a; hspa9-b; Hspa9a; HSPA9B; I79_009139; Mortalin; MOT; Mot-2; MOT2; mt-HSP70; Mthsp70; MTHSP75; PBP74; SAAN; SIDBA4; XELAEV_18020021mg
UniProt ID: (Bovine) Q3ZCH0, (Human) P38646, (Chinese hamster) O35501, (Rat) P48721, (Mouse) P38647
ATP binding
ubiquitin protein ligase binding
nucleotide binding
RNA binding
protein binding
hydrolase activity
ATPase activity
enzyme binding
heat shock protein binding
protein binding involved in protein folding
unfolded protein binding
ATP-dependent protein folding chaperone
fibroblast growth factor binding
poly(A) RNA binding
iron-sulfur cluster assembly
erythrocyte differentiation
regulation of erythrocyte differentiation
negative regulation of erythrocyte differentiation
negative regulation of hematopoietic stem cell differentiation
negative regulation of hemopoiesis
protein folding
protein export from nucleus
response to toxic substance
cellular response to interleukin-1
intracellular protein transport
inner mitochondrial membrane organization
calcium ion transmembrane import into mitochondrion
protein refolding
positive regulation of apoptotic process
negative regulation of apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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