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Invitrogen
CHRNA1 Monoclonal Antibody (88B)
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FIGURE: 1 / 6
CHRNA1 Antibody (MA3-043) in ICC/IF
Immunofluorescent analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) (Product # MA3-043) shows staining in Neuro-2a Cells. Nicotinic Acetylcholine Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Nicotinic Acetylcholine Receptor (Product # MA3-043) at a dilution of 1:100 o... View More
Product Details
MA3-043
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Amphibian,
Chicken,
Fish,
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
88B
Immunogen
Purified Torpedo californica acetylcholine receptor.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-043 detects nicotinic acetylcholine receptor (nAChR) gamma and delta subunits in torpedo and the delta subunit in mouse, human, rat, chicken and frog. This antibody does not detect the alpha 1 and beta 1 subunits.
MA3-043 has been successfully used in Western blot, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 60 kDa protein representing the gamma subunit and a 65 kDa protein representing the delta subunit from torpedo skeletal muscle homogenates. Under non-reducing conditions the delta subunit migrates mostly as a dimer of ~130 kDa. Immunohistochemical staining of nAChR in rat skeletal muscle with MA3-043 results in strong staining of the motor endplate.
In Immunohistochemical staining, cryostat or permeabilization of the sections is recommended due to the reactivity of this antibody with the cytoplasmic side of the receptor.
The MA3-043 antigen is purified Torpedo californica acetylcholine receptor.
Target Information
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine.The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1's, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetylcholine receptor; Acetylcholine receptor subunit alpha; acetylcholine receptor, nicotinic, alpha 1 (muscle); alpha-1 subunit, nicotinic acetylcholine receptor; cholinergic receptor; cholinergic receptor nicotinic alpha polypeptide 1; cholinergic receptor, nicotinic alpha 1; cholinergic receptor, nicotinic, alpha 1 (muscle); cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle); muscle nicotinic acetylcholine receptor; nAChR; nicotinic acetylcholine receptor alpha subunit; nicotinic acetylcholine receptor alpha subunit|AChR alpha subunit; nicotinic cholinergic receptor alpha 1; unnamed protein product
Gene Aliases: Achr-1; ACHRA; ACHRD; Acra; AI385656; AI608266; CHNRA; CHRNA; CHRNA1; CMS1A; CMS1B; CMS2A; FCCMS; NACHRA1; SCCMS
UniProt ID: (Chicken) P09479, (Human) P02708, (Mouse) P04756, (Rat) P25108
Entrez Gene ID: (Chicken) 395608, (Human) 1134, (Mouse) 11435, (Rat) 79557
transmembrane signaling receptor activity
acetylcholine-gated cation channel activity
neurotransmitter receptor activity
ion channel activity
extracellular ligand-gated ion channel activity
acetylcholine receptor activity
acetylcholine binding
transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential
acetylcholine-activated cation-selective channel activity
protein binding
ligand-gated ion channel
signal transduction
chemical synaptic transmission
ion transmembrane transport
regulation of membrane potential
neurological system process
excitatory postsynaptic potential
skeletal muscle contraction
ion transport
cation transport
synaptic transmission, cholinergic
neuromuscular synaptic transmission
neuromuscular junction development
neuronal action potential
response to nicotine
muscle cell cellular homeostasis
skeletal muscle tissue growth
musculoskeletal movement
neuromuscular process
membrane depolarization
regulation of postsynaptic membrane potential
neuron cellular homeostasis
acetylcholine receptor signaling pathway
presynaptic modulation of chemical synaptic transmission
transport
cation transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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