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Histone H2A Monoclonal Antibody (10HH2-2G12)
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Histone H2A Antibody (MA3-047) in WB
Western blot analysis of Histone H2A was performed by loading 20 µg of HeLa soluble nuclear and HeLa chromatin bound cell lysate fractions prepared using a Subcellular Protein Fractionation Kit (Product # 78840) and 5 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. Histone H2A was detected using a Histone... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-047
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
10HH2-2G12
Immunogen
Synthetic peptide: VLLPKKTESH corresponding to amino acids 115-124 of human H2A.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-047 detects Histone H2A in Human samples.
MA3-047 has been succesfully used in western blot and ELISA applications.
Immunogen is a synthetic peptide conjugated to ovalbumin corresponding to residues 115-124 of Human Histone H2A, which is highly conserved across multiple species.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Acetyl-Histone H2; H2A 615 antibody; H2A GL101 antibody; H2A histone family member; H2A histone family member X; H2A histone family member Z; H2A histone family, member A; H2A histone family, member C; H2A histone family, member E; H2A histone family, member G; H2A histone family, member M; H2A histone family, member P; H2A histone family, member R; H2A.1; H2A.1 antibody; H2A/d; h2ad; H2ae; H2AFX; H2AFZ; h2ai; H2ak; H2AK5ac; H2AX histone; H2AZ histone; H2B histone family, member U, (testis-specific); HIS; HIST1H2AE antibody; HIST1H3D; HIST3H2A; histone (H2A.Z); histone 1, H2aa; histone 1, H2ab; histone 1, H2ad; histone 1, H2ae; histone 1, H2ag; histone 1, H2ai; histone 1, H2aj; histone 1, H2ba; histone cluster 1 H2A family member a; histone cluster 1 H2A family member b; histone cluster 1 H2A family member d; histone cluster 1 H2A family member e; histone cluster 1 H2A family member g; histone cluster 1 H2A family member h; histone cluster 1 H2A family member i; histone cluster 1 H2A family member j; histone cluster 1 H2B family member a; histone cluster 1, H2aa; histone cluster 1, H2ab; histone cluster 1, H2ad; histone cluster 1, H2ae; histone cluster 1, H2ag; histone cluster 1, H2ah; histone cluster 1, H2ai; histone cluster 1, H2ba; histone H2A.3; histone H2A.x; histone H2A/a; histone H2A/g; histone H2A/m; histone H2A/p; histone H2A/ptl; histone H2A/r; histone H2A/s; histone H2AD; histone H2AE; histone H2B, testis; MGC74460 antibody; testis-specific histone H2B; unnamed protein product
Gene Aliases: bA317E16.2; bA317E16.3; dJ160A22.4; dJ86C11.1; H2A.1; H2A.1b; H2A.2; H2A.3; H2A.X; H2A.z; H2A.Z-1; H2A/a; H2A/c; H2A/E; H2A/g; H2A/m; H2A/p; H2A/S; H2A/X; H2A/z; H2AA; H2AFA; H2AFALii; H2AFC; H2AFE; H2AFG; H2AFM; H2AFP; H2AFR; H2AFX; H2AFZ; H2AG; H2AH; H2AZ; H2BFU; HIST1H2AA; HIST1H2AB; HIST1H2AD; HIST1H2AE; HIST1H2AG; HIST1H2AH; HIST1H2AI; HIST1H2AJ; HIST1H2BA; HISTH2AA; hTSH2B; pH2A/f; STBP; TH2A; TH2B; TSH2B; TSH2B.1
UniProt ID: (Human) Q96QV6, (Human) P0C0S8, (Human) Q96KK5, (Human) Q99878, (Human) P04908, (Human) P20671, (Human) P16104, (Human) P0C0S5, (Human) Q96A08
Entrez Gene ID: (Human) 221613, (Human) 8969, (Human) 85235, (Human) 8329, (Human) 8331, (Human) 8335, (Human) 3013, (Human) 3012, (Human) 3014, (Human) 3015, (Human) 255626
DNA binding
structural constituent of chromatin
protein heterodimerization activity
protein binding
enzyme binding
damaged DNA binding
histone binding
chromatin-protein adaptor activity
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
chromatin DNA binding
nucleosomal DNA binding
heterochromatin assembly
chromatin organization
positive regulation of transcription from RNA polymerase II promoter
cellular response to estradiol stimulus
negative regulation of cell proliferation
protein localization to CENP-A containing chromatin
nucleosome assembly
nucleosome disassembly
sperm chromatin condensation
DNA damage checkpoint
double-strand break repair
cellular response to DNA damage stimulus
response to ionizing radiation
positive regulation of DNA repair
protein localization to site of double-strand break
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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