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Invitrogen
PTP1B Monoclonal Antibody (OTI1A2)
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FIGURE: 1 / 7
PTP1B Antibody (MA5-25574) in ICC/IF
Immunofluorescence analysis of PTP1B was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 60 minutes at room temperature. The cells were labeled with PTPB1 Monoclonal Antibody (Product # MA5-25574) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175)... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-25574
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI1A2
Immunogen
Full length human recombinant protein of PTPN1 produced in HEK293T cell
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.72 mg/mL
Amount
72 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
PTP1B (PTPN1) is a tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. It mediates dephosphorylation of EIF2AK3/PERK and in turn inactivates the protein kinase activity of EIF2AK3/PERK. PTP1B also plays a role in CKII- and p60c-src-induced signal transduction cascades. It regulates the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. PTP1B also regulates the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CPTP1; EC 3.1.3.48; hgnc9642; KIAA0283; phosphotyrosyl-protein phosphatase (EC 3.1.3.48); protein tyrosine phosphatase, placental; protein-tyrosine phosphatase 1B; PTN1; RP5-1121H13.2; RPTPrho; tyrosine phosphatase 1b; tyrosine-phosphatase; unnamed protein product
Gene Aliases: PTP1B
UniProt ID: (Human) P18031
Entrez Gene ID: (Human) 5770
RNA binding
phosphoprotein phosphatase activity
protein tyrosine phosphatase activity
non-membrane spanning protein tyrosine phosphatase activity
insulin receptor binding
protein binding
zinc ion binding
phosphatase activity
enzyme binding
protein kinase binding
receptor tyrosine kinase binding
cadherin binding
ephrin receptor binding
protein phosphatase 2A binding
positive regulation of systemic arterial blood pressure
negative regulation of cell proliferation
regulation of signal transduction
negative regulation of signal transduction
positive regulation of heart rate
positive regulation of cardiac muscle cell apoptotic process
negative regulation of cell-substrate adhesion
negative regulation of neuron projection development
actin cytoskeleton organization
regulation of endocytosis
regulation of proteolysis
negative regulation of vascular endothelial growth factor receptor signaling pathway
endoplasmic reticulum unfolded protein response
response to nutrient levels
cellular response to insulin stimulus
regulation of intracellular protein transport
cellular response to unfolded protein
response to endoplasmic reticulum stress
peptidyl-tyrosine dephosphorylation
platelet-derived growth factor receptor-beta signaling pathway
cellular response to platelet-derived growth factor stimulus
IRE1-mediated unfolded protein response
insulin receptor recycling
negative regulation of MAP kinase activity
cellular response to fibroblast growth factor stimulus
positive regulation of JNK cascade
regulation of insulin receptor signaling pathway
negative regulation of insulin receptor signaling pathway
negative regulation of protein kinase B signaling
regulation of type I interferon-mediated signaling pathway
JAK-STAT cascade involved in growth hormone signaling pathway
negative regulation of ERK1 and ERK2 cascade
cellular response to hypoxia
cellular response to nitric oxide
vascular endothelial cell response to oscillatory fluid shear stress
regulation of postsynapse assembly
regulation of hepatocyte growth factor receptor signaling pathway
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of IRE1-mediated unfolded protein response
negative regulation of PERK-mediated unfolded protein response
cellular response to angiotensin
negative regulation of vascular associated smooth muscle cell migration
cellular response to nerve growth factor stimulus
response to angiotensin
positive regulation of endothelial cell apoptotic process
positive regulation of receptor catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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