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Invitrogen
ATG16L1 Recombinant Rabbit Monoclonal Antibody (5N2N9)
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FIGURE: 1 / 1
ATG16L1 Antibody (MA5-55248) in WB
Western Blot analysis of various lysates using ATG16L1 Recombinant Monoclonal Antibody (Product # MA5-55248) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-55248
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5N2N9
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 6-105 of human ATG16L1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
Product Specific Information
Immunogen Sequence: RAADFPRWKR HISEQLRRRD RLQRQAFEEI ILQYNKLLEK SDLHSVLAQK LQAEKHDVPN RHEISPGHDG TWNDNQLQEM AQLRIKHQEE LTELHKKRGE
Target Information
Autophagy related protein 16 L is encoded by the ATG16L gene and is located on chromosome 2 in humans. It is also known as WD30 or IBD10 (Inflammatory Bowel Disease 10). ATG 12 and ATG5 conjugate with each other and undergoes homo oligomerisation with ATG16L to form an 800 kDa complex called the Atg16L complex. This complex facilitates coupling or lipidation of LC3 to phosphatidylethanolamine (PE) and this ubiquitin-like conjugation system is a widely used marker for autophagy. However, the ATG 16L complex along with other ATGs like ATG 18 and ATG 21 prevent the premature cleavage of ATG8-PE by the cysteine protease, Atg4 until the autophagosome is deemed complete.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A16L1; APG 16-L1; APG16-like 1; APG16L beta; APG16L isoforms 1; atg16; ATG16 autophagy related 16-like 1; ATG16L1; Atg16L1 gamma; autophagy-related 16-like 1 gamma; Autophagy-related protein 16-1; hCG_1817841; isoform 2 is encoded by transcript variant 2; unnamed protein product; WD repeat domain 30
Gene Aliases: 1500009K01Rik; APG16L; ATG16A; ATG16L; ATG16L1; IBD10; UNQ9393/PRO34307; WDR30
UniProt ID: (Human) Q676U5, (Mouse) Q8C0J2
Entrez Gene ID: (Human) 55054, (Mouse) 77040
autophagosome assembly
transport
autophagy
protein transport
negative stranded viral RNA replication
C-terminal protein lipidation
positive regulation of autophagy
macroautophagy
lysosomal microautophagy
hippocampus development
corpus callosum development
protein localization to pre-autophagosomal structure
defense response to virus
xenophagy
axonal transport
dendrite arborization
negative regulation of dendrite extension
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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