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Invitrogen
Glypican 3 Recombinant Mouse Monoclonal Antibody (8D11)
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FIGURE: 1 / 1
Glypican 3 Antibody (MA5-60712) in Flow
Flow cytometry of Glypican 3 in THP-1 cells. Samples were incubated with Glypican 3 Recombinant Monoclonal antibody (Product # MA5-60712) using a dilution of 1:100 (red line) followed by FITC-conjugated Goat Anti-Mouse IgG(H+L) at a 1:200 dilution for 35 min at 4°C. 10% normal goat serum was incubated to block non-specific protein-protein interactions followed by the antibody (1 µg/10^6 cells) for 45 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of >10, 010 ev... View More
Product Details
MA5-60712
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
8D11
Immunogen
Recombinant Human GPC3 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell surface protein; precursor core protein cleaved by furin; glypican proteoglycan 3; Glypican-3; GTR2-2; GTR22; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI-5; secreted glypican-3; unnamed protein product
Gene Aliases: DGSX; GPC3; GTR2-2; MXR7; OCI-5; OCI5; SDYS; SGB; SGBS; SGBS1
UniProt ID: (Human) P51654
Entrez Gene ID: (Human) 2719
branching involved in ureteric bud morphogenesis
kidney development
smoothened signaling pathway
negative regulation of cell proliferation
response to bacterium
anatomical structure morphogenesis
organ morphogenesis
anterior/posterior axis specification
regulation of signal transduction
body morphogenesis
cell migration
bone mineralization
osteoclast differentiation
lung development
positive regulation of BMP signaling pathway
embryonic hindlimb morphogenesis
regulation of growth
cell migration involved in gastrulation
positive regulation of protein catabolic process
positive regulation of endocytosis
negative regulation of smoothened signaling pathway
positive regulation of smoothened signaling pathway
negative regulation of growth
positive regulation of glucose import
epithelial cell proliferation
negative regulation of epithelial cell proliferation
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
regulation of canonical Wnt signaling pathway
coronary vasculature development
cell proliferation involved in kidney development
mesenchymal cell proliferation involved in ureteric bud development
mesonephric duct morphogenesis
cell proliferation involved in metanephros development
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
regulation of protein localization to membrane
regulation of non-canonical Wnt signaling pathway
positive regulation of Wnt signaling pathway, planar cell polarity pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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