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Invitrogen
Ferritin Monoclonal Antibody (321B7)
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FIGURE: 1 / 4
Ferritin Antibody (MIF2505) in ICC/IF
Immunofluorescent analysis of Ferritin (FTL, green) in HepG2 cells and MCF7 cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with a Ferritin monoclonal antibody (Product # MIF2505), at a dilution of 1:200 for at least 1 hour at room temperature, and then incubated with a DyLight 488 goat anti-mouse secondary antibody (Product # 35502) at a dilution of 1:500 for 30 minu... View More
Product Details
MIF2505
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
321B7
Immunogen
Human liver ferritin
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
5 mg/mL
Amount
1 mg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MIF2505 detects Ferritin in ELISA, IP, IF, and RIA applications and shows reactivity to human samples. The MIF2505 Immunogen is human liver ferritin. MIF2505 detects ferritin which has a predicted molecular weight of approximately 19kDa.
MIF2505 was formerly sold as a Seradyn product.
Target Information
Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. Ferritin is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in the light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. The ferritin gene has multiple pseudogenes, and ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), and capable of storing up to 4,500 atoms of ferric iron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoferritin; Cell proliferation-inducing gene 15 protein; epididymis secretory sperm binding protein; Ferritin H subunit; Ferritin heavy chain; ferritin heavy subunit; Ferritin L subunit; ferritin L-chain; Ferritin light chain; ferritin light polypeptide-like 3; ferritin light subunit; ferritin, heavy polypeptide 1; ferritin, light polypeptide; frih; fril; FTL; H-ferritin; iron ion binding; iron-binding; immunosuppressor; growth factor; L apoferritin; MGC71996; PIG15; placenta immunoregulatory factor; proliferation-inducing protein 15; unnamed protein product
Gene Aliases: FHC; FTH; FTH1; FTHL6; FTL; FTL1; HFE5; LFTD; NBIA3; NBIA9; OK/SW-cl.84; PIG15; PLIF
UniProt ID: (Human) P02794, (Human) P02792
Entrez Gene ID: (Human) 2495, (Human) 2512
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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