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Invitrogen
Zap-70 Polyclonal Antibody
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FIGURE: 1 / 1
Zap-70 Antibody (PA1-19390) in WB
Western blotting analysis of human ZAP70 using rabbit polyclonal antibody PAb (430) (Product # PA1-19390) on lysates of MOLT-4 cell line and HEK293T/17 cell line (ZAP70 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 5µg/mL of rabbit anti-ZAP70 polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for ZAP70 protein at approximately 70kDa.
Product Details
PA1-19390
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 with histidine tag
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes C-terminal part of human ZAP70 protein tyrosine kinase (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Western Blot: Reducing conditions.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; kinase ZAP70; Selective T cell defect; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; unnamed protein product; ZA70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Gene Aliases: ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70; ZAP70
UniProt ID: (Human) P43403
Entrez Gene ID: (Human) 7535
adaptive immune response
immune system process
protein phosphorylation
immune response
leukocyte cell-cell adhesion
peptidyl-tyrosine phosphorylation
calcium-mediated signaling
T cell differentiation
intracellular signal transduction
T cell activation
B cell activation
beta selection
positive regulation of MAPK cascade
positive thymic T cell selection
negative thymic T cell selection
thymic T cell selection
positive regulation of T cell differentiation
alpha-beta T cell differentiation
positive regulation of alpha-beta T cell differentiation
positive regulation of alpha-beta T cell proliferation
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
leukocyte migration
T cell aggregation
T cell migration
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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