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Catenin alpha-1 Polyclonal Antibody
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FIGURE: 1 / 4
Catenin alpha-1 Antibody (PA1-25081) in WB
Western blot was performed using Anti-Catenin alpha-1 Polyclonal Antibody(Product # PA1-25081) and a 100kDa band corresponding to Catenin alpha-1 was observed across cell lines and tissue extracts tested except PC-3 and Daudi which is reported to be negative. A non-specific band around 160kDA has also been observed in cell lines. Whole Cell Extract-WCL (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), LNCaP (Lane 4), PC-3 (Lane 5), Daudi (Lane 6), NIH/3T3 (Lane 7), PC-12 (Lane 8) and tissue extracts of Mouse Lung (Lane 9), Mo... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-25081
Applications
Tested Dilution
Publications
Product Specifications
Published species
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region located near the C-terminus of human alpha-E-catenin (amino acids 873-887).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: A431 and cytosolic fraction of rat embryonic brain, MDCK, MCF-7.
PA1-25081 is expected to cross react with mouse (100% conserved) and Xenopus laevis (100% conserved) due to sequence homology.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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